ESTABLISHMENT OF NEST SEARCH PLOTS
Number and Size of Plots per Site
Each geographic location and habitat condition or type where an
independent study is being conducted will be referred to as a
SITE. At each site, a sufficient number of nest plots should
be established to find at least 20 nests per treatment/habitat type
each year, for each of the most locally common species. In eastern
hardwood forest this is typically about eight 35--50 ha plots,
while in more productive (e.g., western riparian) sites, four 10--20
ha sites might be sufficient. Estimates of nesting success based on
fewer than 20 nests may not be reliable (Hensler and Nichols 1981).
When setting up a new BBIRD site, location of plots must be identified
using a random or stratified random selection process, although
constrained sampling may be used to control for aspect, slope, distance
to roads, or other similar effects. Great caution must be exercised
in terms of multiple objectives. Incorporation of additional
treatments (fragments, land management programs, grazing, etc.) can
fragment sample sizes. Additional objectives should be addressed by
adding nest plots rather than by fragmenting the sample.
There are few specific requirements for plot size and number, but
the following guidelines should be followed when determining the size
and number of plots:
- Nest plots should be a minimum of 200 x 200 m (4 ha) in size
because point count survey locations need to
be completely
nested within the area searched for nests (survey points should be
at least 100 m from the edge of the nest search plot). Small plots
are attractive because a larger geographic area can be sampled but
search efficiency declines as travel time increases between plots.
- Large numbers of nests can be found in a big plot and search
efficiency increases up to a point, but the number of plots that
can be searched and monitored drops rapidly as plot size increases.
The percentage of nests found also declines in large plots, so
there is a trade-off between search efficiency and search
intensity. The potential for site-specific bias increases when
only a few plots are sampled, reducing the power of inference to
areas beyond the plots. It can also be difficult to fit large
plots into a single habitat type. Moreover, when plots represent
treatments (i.e. grazed vs. ungrazed plots), a sufficient number
of plots are necessary to get within and between treatment
variance estimates.
Choosing Plots
Plots must be chosen using a random-selection procedure so that
inferences can be made to the entire site from the data collected.
However, stratifying your site with respect to important features can
improve precision. Obviously habitat type has a strong effect on the
bird community, but within a general habitat type (e.g., Ponderosa
pine) there are many other factors that influence habitat suitability.
For example, aspect and proximity to roads or other anthropogenic
factors can also influence species composition, density, and
productivity. Before selecting plots, give careful thought to factors
that might influence bird productivity in your area. Prior to actual
choice of plot locations, your randomization scheme should be approved
by BBIRD staff. In general, plots should be oriented to maximize local
heterogeneity within the plot by running the long axis of each plot
perpendicular to any obvious habitat gradient. This will minimize
variance among plots.
Also, try to the extent possible to spatially separate the plots
from each other, in order that the plots can be treated as independent
sampling units. Separate plots cannot share a common boundary.
Plot Shape
Plots can be of any shape, but the borders should be well defined
so the area searched for nests can be accurately determined. Plots
should be large enough to fit bird survey points
(50 m fixed radius
plots) completely within the area searched for nests.
Survey points should be separated by 200m and all points should be
at least 100 m from the edge of the plot (minimum plots width = 200 m;
see figure, below).
FIGURE: PLOT SHAPES AND LOCATIONS OF POINT COUNTS

Marking Plots
Permanent markers should be established at 50 m intervals in a
grid system to provide reference points for relocating nests; you may
want to add additional markers between the 50 m marks if vegetation is
dense and distant markers difficult to see. Permanent grid markers
are hereafter referred to as stations. Station markers are
used as field references for relocating nests, but are also used as
reference points for calculating nest locations as UTM coordinates for
use with GIS systems. All cooperators should try and gain the use of a
GPS (Global Positioning System) unit to accurately locate their plots.
(We do have limited access to some GPS units -- contact Tom Martin if
you need a unit.) The GPS units we have access to have a minimum error
ranges of +/- 10 meters. This is great for locating plots within a
larger geographic area but is not sufficient to accurately locate
stations within a plot. We suggest GPS units should be used to
locate 1 station on each plot: UTM locations of all other stations can
then be calculated from distance and compass bearings from the measured
point. Nest locations can be calculated in a similar way using
distance and bearings from known station locations. Please note that
station locations, and hence nest locations, will only be as accurate
as the survey work used to establish stations in the first place.
With good survey work, extrapolating from one GPS location per plot
will result in more internally accurate locations than locating each
station with the GPS unit.
It is important that stations be well marked, and that they survive
between years. If possible, all stations should be permanently marked
with wooden or metal stakes. Be sure to get permission from the
appropriate landowners or agencies before establishing these permanent
markers.