Flourescence Cytometry Resources

Protocols & Resources:

• Titration Protocol (PDF)
• Optimizing Experiments – Tips on Experiment Design (PDF)
• Flow Cytometry Worksheet (PDF)
• Flow Cytometry Worksheet Example (PDF)
• Introduction to Flow Cytometry: A Learning Guide (PDF)

Spectral Viewers:

• http://bdbiosciences.com/spectra/
• http://probes.invitrogen.com/resources/spectraviewer/

Useful Websites:

• www.cyto.purdue.edu/hmarchiv/cytomail.htm the ultimate flow go-to source including a discussion group and many tutorials
• www.bdbiosciences.com/immunocytometry_systems/support/training/online/ online tutorials for flow cytometry and DIVA software
• http://probes.invitrogen.com/resources/education/ a very good tutorial on flow cytometry and fluorescence
• http://facs.scripps.edu/recovery.html for a calculator to help estimate sort recovery
• http://jcsmr.anu.edu.au/facslab/statistics.html for a good tutorial on when to use which statistic for analysis
• www.microbiology.emory.edu/altman/f_protocols/f_flowCytometry/p_titering_Abs.html for an explanation of how and why to titer antibodies
• www.drmr.com for protocols on making your own conjugated antibodies and a great tutorial on compensation

Spectrum:

List of Common Fluorochromes & Dyes detectable by instrument:

There are many other fluorochromes and dyes that can be detected.  Check with one of the spectral viewers listed under Instruments section or check with the Core Facilitator.