Flourescence Cytometry Resources
Protocols & Resources:
- Titration Protocol (PDF)
- Optimizing Experiments – Tips on Experiment Design (PDF)
- Flow Cytometry Worksheet (PDF)
- Flow Cytometry Worksheet Example (PDF)
- Introduction to Flow Cytometry: A Learning Guide (PDF)
Spectral Viewers:
Useful Websites:
- www.cyto.purdue.edu/hmarchiv/cytomail.htm the ultimate flow go-to source including a discussion group and many tutorials
- www.bdbiosciences.com/immunocytometry_systems/support/training/online/ online tutorials for flow cytometry and DIVA software
- http://probes.invitrogen.com/resources/education/ a very good tutorial on flow cytometry and fluorescence
- http://facs.scripps.edu/recovery.html for a calculator to help estimate sort recovery
- http://jcsmr.anu.edu.au/facslab/statistics.html for a good tutorial on when to use which statistic for analysis
- www.microbiology.emory.edu/altman/f_protocols/f_flowCytometry/p_titering_Abs.htm for an explanation of how and why to titer antibodies
- www.drmr.com for protocols on making your own conjugated antibodies and a great tutorial on compensation
Spectrum:
There are many other fluorochromes and dyes that can be detected. Check with one of the spectral viewers listed under Instruments section or check with the Core Facilitator.