Professor Hashim Saber
Department of Mathematics
Montana State University
The Inverse Problem of
Parameter/Protein Identification
Using the Ultracentrifuge
Sedimentation Velocity Experiment
Sedimentation velocity experiment is a widely used method to analyze biochemical systems using the analytical ultracentrifuge. This instrument subjects a small volume of solution to a precisely controlled centrifugal force, and records by means of self-contained and photographic systems the concentration distribution produced in the solution. The problem of estimating the parameters associated with the solution is an inverse problem where the (unknown) parameters are determined from the experimental concentration data of the solution (Protein or DNA) generated by the ultracentrifuge. Those parameters are used to analyze the system in obtaining critical information such as composition, molecular weight, identification of multiple components, and determination of binding coefficients of interacting macromolecules.
The problem, its numerical solution, and the developed software will be discussed.
Thursday, 19 October 2000
4:10 p.m. in Math 109
Coffee/treats at 3:30 p.m. Math 104 (Lounge)
Fall 2000 Colloquium Schedule | Mathematical Sciences home | The University of Montana home