Kathryn Heivilin, CPIA, RBP
Senior IACUC Manager
UM Biosafety Officer
Sentinel Health Program
Last Review Date: September 28, 2020
The purpose of this document is to describe the sentinel program designed to monitor the health status of animals housed in LAR in an effort to prevent, detect and control the presence of specific infectious pathogens which may adversely affect animal health and/or influence research protocols. Sentinels cannot detect all pathogens but may serve as indicators of adherence to and effectiveness of barrier systems, containment areas, and preventive practices such as cage opening/changing, animal transport and procurement, use of protective equipment, and
the SPF mouse facility (SB). The program employs semiannual serologic analysis
MVM, MNV, MPV, Sendai, TMEV, EDIM, and Mycoplasma
(first week of April), we test for these same agents as well as PVM, Reo3, LCM, and
tape) and endoparasite examination (perianal cellophane tapes for Syphacia
examination of cecal and proximal colonic contents for adults of both species)
are conducted semiannually on sentinel mice as well. Blood is collected
optical microscope, depending on the testing methodology.
monitored semiannually for Syphacia muris using the cellophane tape test.
Serology is performed semiannually on extra rats from the primary population
Extra rats used for serology are necropsied and visual examination of cecal
Tyzzer’s disease, Treponema, and Encephalitozoon
D. Guinea pigs
guinea pigs in the animal facility. We do not house guinea pigs long-term in a
breeding or long-term experimentation situation at this time. If this situation
changes, we will expect to monitor semiannually for Sendai, PVM, PI3, and
Encephalitozoon cuniculi while adding once annually to this screen SV5, LCM and
E. Dwarf Hamsters
tested semiannually for Sendai, LCMV and Clostridium piliforme.
used for the degus.
G. Quarantine Sentinel Procedures
be quarantined for 6 weeks in a quarantine cubicle in HSB 004. Sentinel mice
H. Grain Mite Surveillance
infestation. Clear tape is stuck on the bottom inside of each barrel liner