Injection Routes, Needle Sizes, Volumes


Date Adopted: April 10, 2018


INJECTION ROUTES, MAXIMUM NEEDLE SIZES, AND VOLUMES

Animal Species Intradermal Injection Intramuscular Indection Intraperitoneal Injection
Mouse
Skin,
volume: <0.05 ml
Needle size: <25 Ga
Rear leg/thigh,
vol: <0.05 ml
Needle size: <23 Ga
Abdomen, lower quad,
vol: <2-3 ml
Needle size: <21 Ga
Degu
Skin,
volume: <0.05 ml
Needle size: <25 Ga
Rear leg/thigh,
vol: <0.3 ml
Needle size: <21 Ga
Abdomen, lower quad,
vol: <5-10 ml
Needle size: <21 Ga
Rat
Skin,
volume: <0.05 ml
Needle size: <25 Ga
Rear leg/thigh,
vol: <0.3 ml 
Needle size: <21 Ga
Abdomen, lower quad,
vol: <5-10 ml
Needle size: <21 Ga
Hamster
Skin,
volume: <0.05 ml
Needle size: <25 Ga
Rear leg/thigh,
vol: <0.1 ml
Needle size: <21 Ga
Abdomen, lower quad,
vol: <5-10 ml
Needle size: <21 Ga
Guinea Pig
Skin,
volume: <0.05 ml
Needle size: <25 Ga
Rear leg/thigh,
vol:<0.3 ml
Needle size: <21 Ga
Abdomen, lower quad,
vol: <10-15 ml
Needle size: <21 Ga
Snowshoe Hare
Skin,
volume: <0.05 ml
Needle size: <25 Ga
Rear leg/thigh,
vol:<0.3 ml
Needle size:<21 Ga
Abdomen, lower quad,
vol: <10-15 ml
Needle size: <21 Ga
Rabbit
Skin,
volume: <0.05 ml
Needle size:<25 Ga
Rear leg/thigh; lumbar,
vol: <0.5 ml
Needle size: <20 Ga
Abdomen, lower quad,
vol: <50-100 ml
Needle size: <21 Ga

 

Animal Species Intravenous Injection Subcutaneous Injection
Mouse

Lateral tail vein,
vol: <0.2 ml   
Needle size: <25 Ga

Back scruff,
vol: <2-3 ml
Needle size: <20 Ga
Degu Jugular or Femoral vein,
vol: <0.3 ml
Needle size: <25 Ga

Back scruff, 
vol: <5-10 ml
Needle size,: <20 Ga
Rat Lateral tail vein,
vol: <0.5 ml 
Needle size: <23 Ga

Back scruff,
vol: <5-10 ml
Needle size: <20 Ga
Hamster Jugular or Femoral vein,
vol: <0.3 ml
Needle size: <25 Ga

Back scruff,
vol: <3-4 ml  
Needle size: <20 Ga
Guinea Pig Saphenous vein,
vol: <0.5 ml
Needle size: <23 Ga

Back scruff,
vol: <5-10 ml
Needle size: <20 Ga
Snowshoe Hare Marginal ear vein,
vol: <1 - 5 ml
Needle size: <25 Ga
Back scruff,
vol: <5-10 ml
Needle size: <20 Ga
Rabbit Marginal ear vein,
vol: <1 - 5 ml
Needle size: <21 Ga

Back scruff,
vol: <30-50 ml
Needle size: <20 Ga

 

GAVAGE NEEDLES - APPROPRIATE SIZE AND VOLUME BY MOUSE WEIGHT 

Mouse Wt (g) Gauge  Lenght (Inches) Ball Diameter (mm) Recommended Volume
~ 1% BW
Up to 14g 24 1 1.25 0.14 ml for 14 g
15-20g 22 1 or 1.5 1.25 0.15-2.0 ml
20-25g 20 1, 1.5 or 3 2.25 0.20-0.25 ml
25-30g 18 1, 1.5 or 2 2.25 0.25-0.30 ml
30-35g 18 2 or 3 2.25 0.30-0.35 ml

Source for recommended volume: The Mouse in Biomedical Research: Normative Biology, Husbandry, and Models (2006).  Source for recommended sizing: Braintree Scientific.