Dosing Strategies for the Antibiotic Baytril

Last Review Date: June 6, 2018

 

I.  Purpose/Scope

The purpose of this Standard Operating Procedure (SOP) is to outline the dosing and administration of antibiotic Baytril™ (enrofloxacin). The administration of ANY treatments must be performed in consultation with the Attending Veterinarian. All medications delivered in food or water must be clearly identified with the treatment that is being administered. All drugs used in animals must be in date.

II.  Policy

It is LAR policy to meet or exceed all federal, state, and local regulations and guidelines and to comply with all institutional policies and procedures as they apply to the use of animals in research.  Personnel must attend any applicable training in animal care and use, occupational health and safety, equipment operation, and SOPs prior to performing activities outlined in this SOP or work under the direct supervision of trained personnel.

III.  Rats

A.  Injection

1.  Signalment: 5-10 mg/kg SQ every 12 hours for 10 days (unless otherwise 
     directed by the AV)

2.  Concentration: 1:10 dilution = 2.27 mg/ml (undiluted = 22.7 mg/kg)

a.  Obtain an empty, sterile vial

b.  Check that the parent vial of Baytril™ (22.7mg/ml) is not expired and 
      solution has not changed appearance.

c.  Clean port of vial with alcohol prior to each withdrawal.

d.  Withdraw 1 cc of Baytril™ and instill into the empty, sterile vial.

e.  Withdraw 9 cc of sterile saline (utilizing aseptic technique as described 
     above).

f.  Instill the sterile saline into vial with the 1 cc of Baytril

g.  Label the vial with the drug name, concentration and date mixed 

3.  Administer the Baytril™ as per the chart below

4.  Withdraw the required drug dosage using a new sterile needle and syringe for 
     each animal and administer subcutaneously

5. Discard the vial of 1:10 diluted Baytril™ after the dosing regimen is completed

Body Weight (grams)

5.0 mg/kg Dose Injection Volume (ml) SQ

10.0 mg/kg Dose Injection Volume (ml) SQ

150 g

0.33 ml

0.66 ml

200 g

0.44 ml

0.88 ml

250 g

0.55 ml

1.10 ml

300 g

0.70 ml

1.30 ml

350 g

0.80 ml

1.50 ml

400 g

0.88 ml

1.80 ml

450 g

1.00 ml

2.00 ml

500 g

1.10 ml

2.20 ml

550 g

1.20 ml

2.40 ml


B.  Drinking Water Administration

1.  Baytril™ may be administered via drinking water

2.  Water must not be chlorinated or acidified (inactivates the drug)

3.  Baytril™ has a bitter taste.  This bitter taste in combination with an ill animal 
     may decrease the volume of water consumed and prevent therapeutic
     blood 
levels of the antibiotic from being reached

4.  Fruit juice (5-10 ml) or a packet of Splenda may be added to the water bottle 
     to improve palatability

5.  Medicated drinking water must be changed weekly or if it gets cloudy

6.  Add 1.9 ml of 22.7 mg/ml Baytril™ to 250 ml of drinking water (8 oz. bottle) or 
     3.7 ml to a 500 ml bottle of drinking water (16 oz. bottle)

C.  Oral Administration

1.  Signalment: Place two tablets of 0.5 mg tablet of Baytril™ in the cage daily for
     10 days

2.  Remove and replace every 24 hours

 IV.  Degu

A.  Dose as per the rats (above)

V.  Mice

A.  Injection

1.  Signalment: 5 mg/kg SQ every 12 hours for 10 days (unless otherwise directed by the AV)

2.  Concentration: 1:10 dilution = 2.27 mg/ml (undiluted = 22.7 mg/kg)

a.  Obtain an empty, sterile vial

b.  Check that the parent vial of Baytril™ (22.7mg/ml) is not expired and 
      solution has not changed appearance.

c.  Clean port of vial with alcohol prior to each withdrawal.

d.  Withdraw 0.1 cc of Baytril™ and instill into the empty, sterile vial.

e.  Withdraw 0.9 cc of sterile saline (utilizing aseptic technique as described 
     above).

f.  Instill the sterile saline into vial with the 0.1cc of Baytril

g.  Label the vial with the drug name, concentration and date mixed 

3.  Administer the Baytril™ as per the chart below

4.  Withdraw the required drug dosage using a new sterile needle and syringe for 
     each animal and administer subcutaneously

5. Discard the vial of 1:10 diluted Baytril™ after the dosing regimen is completed

Body Weight (grams)

5.0 mg/kg Dose Injection Volume (ml) SQ

15 g

0.03 ml

20 g

0.04 ml

25 g

0.06 ml

30 g

0.07 ml

35 g

0.08 ml

40 g

0.09 ml

45 g

0.10 ml


B.  Drinking Water Administration

1.  Baytril™ may be administered via drinking water

2.  Water must not be chlorinated or acidified (inactivates the drug)

3.  Baytril™ has a bitter taste.  This bitter taste in combination with an ill animal 
     may decrease the volume of water consumed and prevent therapeutic
     blood
 levels of the antibiotic from being reached

4.  Fruit juice (5-10 ml) or a packet of Splenda may be added to the water bottle 
     to improve palatability

5.  Medicated drinking water must be changed weekly or if it gets cloudy

6.  Add 1.9 ml of 22.7 mg/ml Baytril™ to 250 ml of drinking water (8 oz. bottle) or 
     3.7 ml to a 500 ml bottle of drinking water (16 oz. bottle)

References:  Antibiotic Administration in the Drinking Water of Mice

C.  Oral Administration

1.  Signalment: Place ½ tablet of 0.5 mg tablet of Baytril™ in the cage daily for 10
     days

2.  Remove and replace every 24 hours

 VI. Snowshoe Hares and Rabbits

A.  Drinking Water Administration

1.  Signalment: 10 mg/kg in water for 10 days

2.  Check that the parent vial of Baytril™ (100 mg/ml) is not expired and the 
     solution has not changed appearance

3.  Clean the port of the parent vial of Baytril™ with alcohol prior to withdrawal

4.  Withdraw 2.0 cc of  100 mg/ml Baytril™ and place in a 1-liter water bottle

5.  Water must not be chlorinated or acidified (inactivates the drug)

6.  Baytril™ has a bitter taste causing an ill animal to decrease the volume of 
     water consumed and prevent therapeutic blood levels of the antibiotic from 
     being reached

7.  Fruit juice (5-10 ml) or a packet of Splenda may be added to the water bottle 
     to improve palatability

8.  Medicated drinking water must be changed weekly or if it gets cloudy

B.  Injection

1.  Signalment: 15 mg/kg subcutaneously twice a day for 10 days

2.  Check that the parent vial of Baytril™ (100 mg/ml) is not expired and the 
     solution has not changed appearance

3.  Clean the port of the parent vial of Baytril™ with alcohol prior to withdrawal

4.  Withdraw the calculated dose from the Baytril™ vial

5.  Replace the needle on the syringe

6.  Clean the port of a bottle of sterile saline with alcohol prior to withdrawal

7.  With the syringe of Baytril™, withdraw the same volume from the sterile saline

8.  Administer subcutaneously