Euthanasia

Last updated: May 3, 2017


I.  Purpose/Scope

This purpose of this Standard Operating Procedure (SOP) is to outline the procedures on euthanasia and to ensure consistency with the AVMA Guidelines for the Euthanasia of Animals: 2013 Edition.

This procedure applies to all personnel involved in euthanasia of animals involved in research projects sanctioned by The University of Montana-Missoula Institutional Animal Care and Use Committee (IACUC).

II.  Policy
It is LAR policy to meet or exceed all federal, state, and local regulations and guidelines and to comply with all institutional policies and procedures as they apply to the use of animals in research. Personnel must attend any applicable training in animal care and use, occupational health and safety, equipment operation, and Standard Operating Procedures prior to performing activities outlined in this SOP or work under the direct supervision of a trained LAR staff member.

III. Animal Identification

It is the responsibility of the person performing euthanasia to verify accurately the identity of the animal(s) to be euthanatized and to ensure that the principal investigator (PI) or designated animal user has authorized euthanasia.  It is imperative to double-check, at a minimum, animal identity before performing euthanasia.

IV. Selection of Specific Agents
The selection of specific agents and methods for euthanasia will depend on the species involved and the objectives of the protocol. Generally, inhalant (non-explosive inhalant anesthetics or CO2) or injectable chemical agents (such as barbiturates) are preferable to physical methods (such as cervical dislocation, decapitation, and use of a penetrating captive bolt). Euthanasia is to be performed by personnel who are skilled in methods for the species in question and in a professional and compassionate manner. Death should be confirmed by personnel who can recognize cessation of vital signs in the species being euthanatized.

V. Non-Explosive Inhalant, CO2
This method is used for rodents and guinea pigs 600 grams and under.

  • Sign-in using clipboard by CO2 unit.  Fill out completely including PI name and number of boxes of animals.
  • Place animal to be euthanatized in the chamber, inside its primary enclosure, if possible.
  • Fill euthanasia chamber with CO2 by turning the tank on.
  • The regulator has been preset for low-flow volume appropriate to the chamber.  DO NOT readjust the brass knob.
  • When no movement is detected, turn off CO2
  • Remove animal and check for cessation of vital signs
  • Use a secondary mechanical means of euthanasia to ensure death (cervical dislocation, decapitation, or creation of pneumothorax).
  • When animal is confirmed dead, place animal in carcass bags (trash bag) and label appropriately (e.g., wild type, transgenic, contaminated)
  • Discard into trash or appropriate carcass freezer ensuring compliance with NIH disposal guidelines
  • Wipe CO2 chamber with Rescue RTU spray after use

VI. IP Pentobarbital euthanasia for rodents
This method is used for rodents and guinea pigs 600 grams and under.

  • Inject animal with an IP overdose of pentobarbital
  • When animal no longer shows any vital signs, use a secondary mechanical means of euthanasia to ensure death (cervical dislocation, decapitation, or creation of pneumothorax).
  • When animal is confirmed dead place animal in carcass bags (trash bag)
  • Discard into trash or appropriate carcass freezer ensuring compliance with NIH disposal guidelines

VII. Injectable Chemical Agents
Chemical agents such as barbiturates are used on larger species such as rabbits, sheep, and non-human primates. Each species requires different dose levels to ensure painless death. It is required that trained personnel perform these procedures, under direct supervision of the Attending Veterinarian (AV) or their supervisor.

  • Check with AV for proper dose requirements. Depending on the research protocol, it may be possible to administer a tranquilizer subcutaneously or intramuscularly 10-15 minutes before injection of euthanasia solution.
  • Use proper needle and syringe size for the species and drug dose volume
  • Euthanasia solution may be obtained from the AV or authorized LAR personnel. All doses must be signed out and logged in the controlled substance log book.
  • Use an easily identifiable vein for injection
  • Place used needle and syringe in sharps container
  • When no movement is detected, check for cessation of breathing and heart beat using a stethoscope
  • When animal is confirmed dead place animal in carcass bags (double bag)
  • Label bag with date, species, and investigator's name
  • Place the bag in the carcass freezer.