Kathryn Mariucci, CPIA, RBP
Senior IACUC Manager
UM Biosafety Officer
Last updated: January 28, 2015
Ivermectin is used as a treatment for mites in mice. The purpose of this SOP is to outline how at apply the Ivermectin solution for treatment of mice.
It is the responsibility of the animal care technician to follow the correct procedures for treating mice that are diagnosed with mites. It is also the responsibility of the animal care technician to report any adverse affects that may result during the period of treatment.
- Mixing the Ivermectin solution
- The technician responsible for spraying the infected mice will be given a spray bottle that contains water and the ivermectin 1/10 dilution.
- The spray bottle that contains the ivermectin should be kept in a brown bag until it is ready to be used. It must be kept in the dark to prevent any alterations of the drug.
- Check the expiration dates on all bottles.
- Spraying the Mice
- Hold spray bottle approximately one (1) inch above the clean cage.
- Spray one pump of fine mist of the ivermectin solution on the mice. You may spray twice if several mice are at opposite ends of the cage. If pinkies are present, ONLY SPRAY ONCE. (NOTE: Once the animals are sprayed they should appear to have a dusting of ivermectin solution on the fur. To prevent ivermectin overdose, NEVER soak an animal with the solution)
- Repeat the process for the other mice needing treatment.
- Return any unused ivermectin to facility manager along with any empty spray bottles.
IV. Safety Considerations
- If eyes are accidentally sprayed, flush thoroughly with plenty of water for at least 15 minutes. Eye wash stations are available in each animal facility.
- If skin comes in contact with the Ivermectin, flush thoroughly with plenty of water and wash contact area with soap and water. Remove contaminated clothing and wash before reuse.
- In case of accidental inhalation overexposure, get to fresh air.
- If ingested, get medical attention.
- If at anytime irritation occurs or persists, get medical attention.
Document the treatment on the animal room check sheet in the comments section.