The University of Montana Genomics Core Laboratory houses an Illumina MiSeq benchtop sequencer.  The MiSeq instrument utilizes a single flowcell and reagent kit in each run. There are a variety of kits and configurations to support any level of sequencing need.  The MiSeq can be used for paired-end as well as single end sequencing.  MiSeq reagent kits v2 (50, 300, and 500 cycle kits) expect sequence from 10-15 million reads (24 to 30 million reads for paired end) and v3 kits (150 and 600 cycle kits) will yield 22-25 million reads (44 to 50 million reads for paired end).

The University of Montana Genomics Core Laboratory contains all the necessary instrumentation for library preparation.

We prepare standard as well as custom libraries including genomic DNA-seq and RNA-seq methods. Access to the laboratory and training on instruments including liquid handling robot is available if you wish to prepare libraries.

The University of Montana Genomics Core Laboratory provides full service cycle sequening of purified DNA template, PCR product, or plasmid.  This includes dye terminator removal, and data generation on the ABI 3130xl genetic analyzer. Sequencing can be done using either standard sequencing primers provided by the core or user provided custom primers. Technical support and troubleshooting is also provided at customers request. Sequence service and sample submission information.

The University of Montana Genomics Core Laboratory is equipped with an Applied Biosystems Genetic Analyzer for use in Fragment Analysis including GeneScan size standards in AFLP, SNP, and MSAT analysis. Fragment analysis service and sample submission information.